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Keywords:

  • Betula pendula;
  • birches;
  • microsatellite;
  • SSR;
  • white birch

Abstract

This paper reports the development of microsatellite primers for a widespread birch species Betula pendula. By screening genomic libraries with (TC)10 and (TG)10 probes, 38 microsatellite sequences were obtained, of which 23 proved to be polymorphic. Thirty genotyped individuals had two to 20 alleles per loci and a heterozygosity range of 0.17–0.92. Cross-species amplification tests of four primer pairs on diverse birch species showed positive results in all cases. Also, one of two loci tested on related genus Alnus gave positive result.