Isolation of six microsatellite markers from the pea aphid, Acyrthosiphon pisum (Homoptera, Aphididae)


Izumi Yao. Fax: +81 11 706 4939; E-mail:


Using the magnetic particles method, we isolated six polymorphic microsatellite loci from the pea aphid, Acyrthosiphon pisum (Harris), which feeds on a wide range of legume species. The isolated loci were polymorphic, with three to six alleles in 40 aphids. Expected heterozygosities ranged from 0.12 to 0.65. These loci can be used to quantify clonal diversity and compare genetic population structure between sexual and asexual populations.