Bluehead wrasse (Thalassoma bifasciatum) are common coral reef fish found throughout the Caribbean. Larval duration in the plankton is relatively long for this species, which raises the possibility of long-distance transport by ocean currents. Several nongenetic studies suggest, however, that most recruits were spawned locally. Determination of the relative importance of local recruitment vs. immigration for recruitment is needed for the effective management of populations and establishment of marine reserves. We characterized seven new polymorphic microsatellite loci for bluehead wrasse and optimized them into PCR multiplexes with three previously published loci to determine the level of dispersal between populations.