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Keywords:

  • dinucleotide repeats;
  • grey fox;
  • microsatellite;
  • polymerase chain reaction;
  • primer;
  • simple sequence repeat;
  • tetranucleotide repeats;
  • Urocyon cinereoargenteus

Abstract

We describe polymerase chain reaction (PCR) primers and conditions to amplify one dinucleotide and five tetranucleotide microsatellite DNA loci isolated from the grey fox (Urocyon cinereoargenteus). The PCR primers were tested on nine to 12 individuals collected from the Department of Energy's Savannah River site (Aiken, SC, USA). The grey fox microsatellite primers developed had three to 10 alleles per locus that yielded observed heterozygosities ranging from 0.222 to 0.889.