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A simple and inexpensive method for producing fluorescently labelled size standard

Authors

  • V. VAUGHAN SYMONDS,

    Corresponding author
    1. University of Texas-Austin, Department of Molecular, Cell, and Developmental Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, MBB 1.448b Austin, TX 78712, USA
      Vaughan Symonds. Fax: 512-232-3432; E-mail: vsymonds@mail.utexas.edu
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  • ALAN M. LLOYD

    1. University of Texas-Austin, Department of Molecular, Cell, and Developmental Biology, Institute for Cellular and Molecular Biology, 2500 Speedway, MBB 1.448b Austin, TX 78712, USA
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Vaughan Symonds. Fax: 512-232-3432; E-mail: vsymonds@mail.utexas.edu

Abstract

Current methods of automated genotyping offer many advantages over traditional gel-based approaches, including reduced handling and processing times, and increased accuracy and consistency. Unfortunately, these advances have come at a substantial cost; at present, roughly one-half of the cost of automated genotyping is due to fluorescently labelled internal size standard. Here we describe detailed methodologies for generating a highly consistent, fluorescently labelled, internal size standard using polymerase chain reaction (PCR). The methods are simple and the required reagents are inexpensive, making the in-house production of fluorescently labelled size standards a more widely accessible alternative to commercially available size standards.

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