Phyto-specific 16S rDNA PCR primers for recovering algal and plant sequences from mixed samples

Authors


Dr John W. Stiller. Fax: 252-328-4178; E-mail: stillerj@mail.ecu.edu

Abstract

Molecular environmental sampling of the phototrophic complexity in a given environment may be important in a number of research disciplines. Because of the broad evolutionary diversity of photosynthetic organisms, however, primers that can recover sequences from all phototrophs also target other organisms, often preferentially. Therefore, PCR primers that selectively amplify genes of phototrophs over those of other prokaryotic and eukaryotic organisms could prove extremely useful. Here we report two such primers that target 16S rDNA from Cyanobacteria and eukaryotic plastids, but do not amplify genes from abundant Bacteria in a mixed sample.

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