Isolation of polymorphic microsatellite markers from the malaria vector Anopheles marajoara (Diptera: Culicidae)

Authors

  • CONG LI,

    1. Department of Entomology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500,
    Search for more papers by this author
  • RICHARD C. WILKERSON,

    Corresponding author
    1. Department of Entomology, Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, 503 Robert Grant Avenue, Silver Spring, MD 20910-7500,
    2. Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103
    Search for more papers by this author
  • DINA M. FONSECA

    1. Academy of Natural Sciences, 1900 Benjamin Franklin Parkway, Philadelphia, PA 19103
    Search for more papers by this author

Richard C. Wilkerson, §Mailing address: Walter Reed Biosystematics Unit Museum Supporting Center, SI, 4210 Silver Hill Rd., Suitland, MD 20746. Tel.: (301) 238 3162; Fax: (301) 238 3168; E-mail: wilkersonr@si.edu

Abstract

Microsatellite-containing regions were isolated and characterized in Anopheles (Nyssorhynchus) marajoara, a primary vector of malaria parasites in northeastern Amazonia, Brazil. An enrichment protocol yielded 500 positive clones. We designed primers to amplify 40 unique microsatellites, 11 of which amplified cleanly and were polymorphic. A survey of 323 individuals showed that these loci are highly variable with the number of alleles ranging from 11 to 52, and expected heterozygosity ranging from 0.64 to 0.95. These markers will be useful for studies of population structure and intraspecific variation in A. marajoara.

Ancillary