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Isolation and characterization of polymorphic microsatellite markers in the gray, short-tailed opossum (Monodelphis domestica)

Authors


Nicolas Gouin. *Present address: Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095–1489, USA, Fax: 210-670-3317; E-mail: ngouin@darwin.sfbr.org

Abstract

Twenty-six polymorphic microsatellite markers were isolated from (AC)n and (AG)n microsatellite-enhanced genomic libraries of the gray, short-tailed opossum Monodelphis domestica. All 26 loci showed high allelic diversity, with allele numbers ranging from five to 11 in a subset of 35 animals. Normal Mendelian inheritance was confirmed for 24 loci by analysing allelic segregation in 10, two-generation, families. Non-amplifying (null) alleles were detected at two loci, which we recommend be used only if pedigree data are available. We conclude that all of these microsatellite markers would be useful for quantitative trait locus mapping and population genetic studies.

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