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Isolation and characterization of microsatellite markers in the tetraploid birch, Betula pubescens ssp. tortuosa


F. Felber, Université de Neuchâtel, Laboratoire de Botanique évolutive, Rue Emile-Argand 11, CH-2007 Neuchâtel, Switzerland. Tel.: +41 32 718 2339; Fax: +41 32 718 3656,


The mountain birch, Betula pubescens ssp. tortuosa is a tetraploid tree species which forms the tree limit in northern Fennoscandia. We identified nine polymorphic microsatellite loci in order to characterize the genetic structure of populations at different elevations close to the treeline. The microsatellite loci were highly polymorphic, with 14–42 alleles per locus and an average expected heterozygosity of 0.73 ± 0.25 under random chromosome segregation and 0.68 ± 0.23 under random chromatid segregation.