Microsatellite loci for Myrmica kotokui and their application in some congeneric ant species distributed in northern Japan

  1. N. AZUMA1,*,
  2. J. TAKAHASHI2,
  3. T. KIKUCHI3,
  4. M. YOSHIMURA4,
  5. K. ONOYAMA5,
  6. S. HIGASHI6

Article first published online: 23 DEC 2004

DOI: 10.1111/j.1471-8286.2004.00849.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 5, Issue 1, pages 118–120, March 2005

Additional Information

How to Cite

AZUMA, N., TAKAHASHI, J., KIKUCHI, T., YOSHIMURA, M., ONOYAMA, K. and HIGASHI, S. (2005), Microsatellite loci for Myrmica kotokui and their application in some congeneric ant species distributed in northern Japan. Molecular Ecology Notes, 5: 118–120. doi: 10.1111/j.1471-8286.2004.00849.x

Author Information

  1. 1

    Laboratory of Animal Cytogenetics, Center for Advanced Science and Technology, Hokkaido University, Sapporo, 060–0810,

  2. 2

    Laboratory of Entomology, Graduate School of Agriculture, Tamagawa University, Machida, Tokyo, 194–8610,

  3. 3

    Laboratory of Entomology, Department of Environmental Science and Technology, Faculty of Agriculture, University of the Ryukyus, Okinawa, 903–0213,

  4. 4

    Institute of Tropical Agriculture, Kyushu University, Fukuoka, 812–8581,

  5. 5

    Obihiro University of Agriculture and Veterinary Medicine, Obihiro, 080–8555,

  6. 6

    Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, 060–0810, Japan

* Noriko Azuma. Fax: +81-138-40-8864; E-mail: anoriko@ees.hokudai.ac.jp

Publication History

  1. Issue published online: 23 DEC 2004
  2. Article first published online: 23 DEC 2004
  3. Received 25 June 2004; revision received 21 July 2004; accepted 15 October 2004

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Keywords:

  • Microsatellite DNA;
  • Myrmica kotokui;
  • Myrmica rubra;
  • polygyny;
  • RAPD

Abstract

We developed seven polymorphic microsatellite loci for Myrmica kotokui from RAPD (rapid analysis of polymorphic DNA) fragments. These loci showed two to six alleles with expected and observed heterozygosity of 0.13–0.73 and 0.14–0.78, respectively. These loci are transferable to the congeneric species as Myrmica rubra, Myrmica luteola and Myrmica taediosa from northern Japan. These loci will allow analyses of genetic structure of Myrmica species at both the colony level and population levels.

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