Microsatellite markers for the large blue butterflies Maculinea nausithous and Maculinea alcon (Lepidoptera: Lycaenidae) and their amplification in other Maculinea species

Authors

  • INGA ZEISSET,

    Corresponding author
    1. Department of Population Biology, Institute of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen Ø, Denmark,
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  • THOMAS DAMM ALS,

    1. Department of Ecology & Genetics, Institute of Biological Sciences, University of Aarhus, building 540, Ny Munkegade, 8000 Aarhus C, Denmark,
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    • §

      Present address: Centre for Basic Psychiatric Research, Psychiatric Hospital in Aarhus, Skovagervej 2, 8240 Risskov, Denmark

  • JOSEF SETTELE,

    1. UFZ — Centre for Environmental Research Leipzig-Halle, Theodor-Lieser-Str. 4, 06120 Halle, Germany
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  • JACOBUS J. BOOMSMA

    1. Department of Population Biology, Institute of Biology, University of Copenhagen, Universitetsparken 15, 2100 Copenhagen Ø, Denmark,
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Inga Zeisset, Fax: 0045 353 21250; E-mail: izeisset@bi.ku.dk

Abstract

We developed microsatellite markers for Maculinea nausithous and Maculinea alcon, two of five species of endangered large blue butterflies found in Europe. Two separate microsatellite libraries were constructed. Eleven markers were developed for M. nausithous and one for M. alcon. The primers were tested on both species as well as on the three other European Maculinea species. The number of alleles per locus ranged from two to 14. These markers will be useful tools for population genetic studies of Maculinea species.

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