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Dinucleotide microsatellite primers designed for a critically endangered primate, the black lion tamarin (Leontopithecus chrysopygus)

Authors

  • BEATRIZ M. PEREZ-SWEENEY,

    Corresponding author
    1. Division of International Medicine and Infectious Diseases, Department of Medicine, Weill Medical College of Cornell University, 1300 York Avenue, New York, NY 10021,
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  • CLAUDIO VALLADARES-PADUA,

    1. University of Brazilia, UNB Colina Bloco Gapto J03, Brazilia DF, Brazil,
    2. Instituto de Pesquisas Ecologicas, Caixa Postal 47, Nazare Paulista, SP, 12960–000, Brazil,
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  • ANDREW S. BURRELL,

    1. Department of Anthropology, New York University, Rufus Smith, 25 Waverly Place, 802/803, New York, NY 10003,
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  • ANTHONY DI FIORE,

    1. Department of Anthropology, New York University, Rufus Smith, 25 Waverly Place, 802/803, New York, NY 10003,
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  • JESSICA SATKOSKI,

    1. Department of Ecology, Evolution, and Environmental Biology (E3B), Columbia University, 1200 Amsterdam Avenue, New York, NY 10027,
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  • PETER J. VAN COEVERDEN DE GROOT,

    1. Department of Biology, Queens University, Kingston, Ontario, Canada, K7L 3 N6,
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  • PETER T. BOAG,

    1. Department of Biology, Queens University, Kingston, Ontario, Canada, K7L 3 N6,
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  • DON J. MELNICK

    Corresponding author
    1. Department of Ecology, Evolution, and Environmental Biology (E3B), Columbia University, 1200 Amsterdam Avenue, New York, NY 10027,
    2. Center For Environmental Research and Conservation (CERC), 10th Floor, Columbia University, 1200 Amsterdam Avenue, New York, NY 10027
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Beatriz Perez-Sweeney, Fax: 212 746 8675; E-mail: bmp8@columbia.edu; Don J. Melnick, Fax: 212 854 8188; E-mail: djm7@columbia.edu

Abstract

Black lion tamarin (BLT) monkeys (Leontopithecus chrysopygus) have suffered a severe reduction in their natural range and are consequently critically endangered. Because allozyme data showed very low levels of variation, it was not clear if these monkeys had much genetic diversity. We designed microsatellite primers for BLTs, and from them we identified nine polymorphic loci, seven of which were tested on golden lion tamarins (GLTs) (Leontopithecus rosalia). All of the seven polymorphic loci and two other monomorphic BLT loci were polymorphic in GLTs. The microsatellite markers identified here are directly applicable to ongoing lion tamarin population and conservation genetics studies.

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