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Characterization and isolation of DNA microsatellite primers for the tropical tree, Symphonia globulifera Linn. f.

Authors

  • C. C. VINSON,

    Corresponding author
    1. Universidade Federal do Pará, Campus de Bragança, Rua Leandro Ribeiro Aldeia, Bragança, Pará, CEP 68600 000, Brazil,
    2. Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte Final, Brazilia, DF, CEP 70770 900, PoBox 02372, Brazil
      C. C. Vinson. E-mail: ccvinson@yahoo.com.br
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  • A. C. AMARAL,

    1. Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte Final, Brazilia, DF, CEP 70770 900, PoBox 02372, Brazil
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  • I. SAMPAIO,

    1. Universidade Federal do Pará, Campus de Bragança, Rua Leandro Ribeiro Aldeia, Bragança, Pará, CEP 68600 000, Brazil,
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  • A. Y. CIAMPI

    1. Embrapa Recursos Genéticos e Biotecnologia, PqEB W5 Norte Final, Brazilia, DF, CEP 70770 900, PoBox 02372, Brazil
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C. C. Vinson. E-mail: ccvinson@yahoo.com.br

Abstract

Five highly polimorphic microsatellite markers for Symphonia globulifera were developed from enriched repeat libraries screened for microsatellite repeats. An average of 5.8 alleles per locus and expected heterozygosity ranging from 0.649 to 0.806 were found in a sample of 12 S. globulifera individuals from the Tapajos National Forest, in the state of Pará in the Brazilian Amazon. These loci are being used for analysis of precise paternity testing, estimation of gene flow and parentage coefficients in a managed forest in Pará, Brazil, as part of the Dendrogene Project.

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