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Isolation and characterization of new microsatellite markers in the surfclam Mactromeris polynyma

Authors

  • MANON C. CASSISTA,

    Corresponding author
    1. Department of Biology, Dalhousie University, 1355 Oxford Street, Halifax, NS B3H 4J1, Canada,
    2. Department of Fisheries and Oceans, Bedford Institute of Oceanography, 1 Challenger Drive, PO Box 1006, Dartmouth, NS B2Y 4A2, Canada,
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  • MICHAEL W. HART

    1. Department of Biology, Dalhousie University, 1355 Oxford Street, Halifax, NS B3H 4J1, Canada,
    2. Department of Biological Sciences, Simon Fraser University, 8888 University Drive, Burnaby, BC V5A 1S6, Canada
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Manon C. Cassista, Fax: 902 426 1862; E-mail: cassistam@mar.dfo-mpo.gc.ca

Abstract

We describe primers and amplification conditions for seven microsatellite loci that were developed and characterized in the commercially harvested surfclam Mactromeris polynyma. Five of these loci were polymorphic: we found 6 to 23 alleles per locus among 100 individuals from one population in Nova Scotia, with some large heterozygote deficits that may reflect unrecognized temporal genetic variation. We are using these markers to investigate temporal and spatial genetic structure in this species.

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