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Keywords:

  • Fundulus catenatus;
  • Fundulus cingulatus;
  • Fundulus confluentus;
  • Fundulus majalis;
  • Fundulus olivaceus;
  • Fundulus seminolis;
  • Fundulus similis

Abstract

The mummichog (Fundulus heteroclitus), a common Atlantic coastal killifish, is a model vertebrate species for the study of molecular genetic variation in natural populations and of environmental toxicology. We report the development of a set of 20 microsatellite loci in this species. Average expected heterozygosity across all loci was 0.84 (range: 0.60–0.97), revealing a high level of variability at most loci. A survey of seven additional Fundulus species yielded one or two robust amplification products in over half (63%) of the species–primer combinations tested. Therefore, many of these loci will also prove useful in studies of other members of the genus Fundulus.