Characterization of nuclear microsatellite loci in South American Araucariaceae species

Authors

  • F. SALGUEIRO,

    Corresponding author
    1. LGMV — Laboratório de Genética Molecular Vegetal, Departamento de Genética, IB, Universidade Federal do Rio de Janeiro — UFRJ, Rio de Janeiro, Brazil,
    2. INRA — UMR BIOGECO, Station de Recherches Forestières, Laboratoire de Génétique des Arbres Forestiers, 33612 CESTAS cedex, France,
      F. Salgueiro, Fax: 25626380; E-mail: fabianos@biologia.ufrj.br
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  • H. CARON,

    1. INRA — UMR BIOGECO, Station de Recherches Forestières, Laboratoire de Génétique des Arbres Forestiers, 33612 CESTAS cedex, France,
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  • M. I. F. DE SOUZA,

    1. LGMV — Laboratório de Genética Molecular Vegetal, Departamento de Genética, IB, Universidade Federal do Rio de Janeiro — UFRJ, Rio de Janeiro, Brazil,
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  • A. KREMER,

    1. INRA — UMR BIOGECO, Station de Recherches Forestières, Laboratoire de Génétique des Arbres Forestiers, 33612 CESTAS cedex, France,
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  • R. MARGIS

    1. LGMV — Laboratório de Genética Molecular Vegetal, Departamento de Genética, IB, Universidade Federal do Rio de Janeiro — UFRJ, Rio de Janeiro, Brazil,
    2. Departamento de Bioquímica, IQ, UFRJ, Rio de Janeiro, Brazil
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F. Salgueiro, Fax: 25626380; E-mail: fabianos@biologia.ufrj.br

Abstract

The Araucariaceae family has only two species in South America: Araucaria angustifolia and Araucaria araucana. Both species are mainly used for timber and have been overexploited in the past. Currently, they are found as fragmented populations and are classified under the International Union for Conservation of Nature and Natural Resources (IUCN) guidelines as vulnerable species. Population fragmentation may seriously affect the genetic diversity of these two species of Araucariaceae and can consequently lead to decreased survival. To better understand the genetic structure of these South American Araucaria species, eight nuclear microsatellites are reported: six new microsatellites loci developed based on a membrane enrichment procedure and two microsatellites loci transferred from the related species, Araucaria cunninghamii.

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