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Seventy-five EST-linked Atlantic salmon (Salmo salar L.) microsatellite markers and their cross-amplification in five salmonid species

Authors

  • ANTI VASEMÄGI,

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    1. Department of Aquaculture, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden,
    2. Institute of Zoology and Hydrobiology, University of Tartu, 51014 Tartu, Estonia,
      Anti Vasemägi, Fax: +4690 123 729; E-mail: anti.vasemagi@vabr.slu.se
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  • JAN NILSSON,

    1. Department of Aquaculture, Swedish University of Agricultural Sciences, S-901 83 Umeå, Sweden,
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  • CRAIG R. PRIMMER

    1. Department of Biological and Environmental Sciences (Biocentre 3), PO Box 65, FIN-00014, University of Helsinki, Finland
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Anti Vasemägi, Fax: +4690 123 729; E-mail: anti.vasemagi@vabr.slu.se

Abstract

An Atlantic salmon (Salmo salar L.) expressed sequence tag (EST) database consisting of 58 146 ESTs was screened for microsatellite sequences. Subsequent development of 75 polymorphic EST-associated microsatellite markers in this species is described together with cross-species amplification results of 133 gene-associated tandem repeat markers in five salmonid species (Salmo trutta, Oncorhynchus mykiss, Salvelinus aplinus, Thymallus thymallus, Coregonus lavaretus). The number of alleles among EST-linked microsatellites in Atlantic salmon ranged from two to 41 with an average of 12 alleles per locus. Cross-species amplification resulted in detection of a total of 111 polymorphic locus-species combinations (12–32 loci per species).

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