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Keywords:

  • Dexter National Fish Hatchery and Technology Center;
  • microsatellites;
  • Plagopterus;
  • warmwater fish;
  • woundfin

Abstract

Woundfin (Plagopterus argentissimus) is one of several endangered warmwater fishes once common to the lower Colorado River basin. Dexter National Fish Hatchery & Technology Center maintains a refugium stock founded in 1982, and augmented with c. 1000 young fish in 1989. Genetic characterization of the refugium stock and salvaged fish from the Virgin River is necessary to develop a strategy to incorporate salvaged fish into the hatchery stock. We developed an enriched microsatellite library and 12 species-specific polymorphic loci. Alleles per locus ranged from 10 to 19, averaging 13. The average expected and observed heterozygosities were 0.884 and 0.895, respectively.