Development and characterization of microsatellite markers for two dioecious Ficus species

Authors

  • MONIKA ZAVODNA,

    Corresponding author
    1. Department of Identity and Genetic Diversity, Plant Research International, Wageningen UR, PO Box 16, 6700 AA Wageningen, The Netherlands,
    2. Department of Plant Population Biology, Netherlands Institute of Ecology (NIOO-KNAW), PO Box 40, 6666 ZG Heteren, The Netherlands
      M. Zavodna, Fax: +31 (0)26 4723227; E-mail: m.zavodna@nioo.knaw.nl or z_monika@hotmail.com
    Search for more papers by this author
  • PAUL ARENS,

    1. Department of Identity and Genetic Diversity, Plant Research International, Wageningen UR, PO Box 16, 6700 AA Wageningen, The Netherlands,
    Search for more papers by this author
  • PETER J. VAN DIJK,

    1. Department of Plant Population Biology, Netherlands Institute of Ecology (NIOO-KNAW), PO Box 40, 6666 ZG Heteren, The Netherlands
    Search for more papers by this author
  • BEN VOSMAN

    1. Department of Identity and Genetic Diversity, Plant Research International, Wageningen UR, PO Box 16, 6700 AA Wageningen, The Netherlands,
    Search for more papers by this author

M. Zavodna, Fax: +31 (0)26 4723227; E-mail: m.zavodna@nioo.knaw.nl or z_monika@hotmail.com

Abstract

Microsatellite markers for Ficus montana and Ficus septica were developed using genomic libraries enriched for di-, tri- and tetranucleotide repeats. The subsets of five and three best scorable primer pairs were characterized on 24 F. montana and 36 F. septica individuals, respectively. For F. montana, loci showed five to 14 alleles per locus and expected heterozygosities ranged between 0.23 and 0.87. For F. septica, loci showed three to five alleles per locus and expected heterozygosities ranged between 0.36 and 0.49. Four primer pairs (two from each subset) cross-amplified in the other species, indicating transportability of the markers within the genus Ficus.

Ancillary