Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae)

  1. ADRIANA OTERO-ARNAIZ1,
  2. ANDREW SCHNABEL1,*,
  3. TRAVIS C. GLENN2,
  4. NANCY A. SCHABLE2,
  5. CRIS HAGEN2,
  6. LEILA NDONG1

Article first published online: 31 MAR 2005

DOI: 10.1111/j.1471-8286.2005.00929.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 5, Issue 2, pages 366–368, June 2005

Additional Information

How to Cite

OTERO-ARNAIZ, A., SCHNABEL, A., GLENN, T. C., SCHABLE, N. A., HAGEN, C. and NDONG, L. (2005), Isolation and characterization of microsatellite markers in the East African tree, Acacia brevispica (Fabaceae: Mimosoideae). Molecular Ecology Notes, 5: 366–368. doi: 10.1111/j.1471-8286.2005.00929.x

Author Information

  1. 1

    Department of Biological Sciences, Indiana University South Bend, PO Box 7111, South Bend, IN 46634, USA,

  2. 2

    Savannah River Ecology Laboratory, Drawer E, Aiken, SC 29802, USA

* Andrew Schnabel, Fax: (574) 520 5589; E-mail: aschnabe@iusb.edu

Publication History

  1. Issue published online: 31 MAR 2005
  2. Article first published online: 31 MAR 2005
  3. Received 28 November 2004; revision accepted 14 January 2005

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Keywords:

  • Acacia;
  • gene flow;
  • genetic structure;
  • molecular markers;
  • PCR;
  • SSR

Abstract

We have developed primer pairs for 24 microsatellite loci isolated from Acacia brevispica, which is widespread in woodland savannas of East Africa. The loci were screened for levels of variation using 16–52 individuals from Mpala Research Centre, Kenya. Number of alleles per locus ranged from two to 17, and polymorphic information content ranged from 0.248 to 0.864. Several loci showed the presence null alleles, but many loci will be useful in ongoing studies of genetic structure, gene flow, breeding systems, and natural selection.

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