Get access

Isolation and characterization of microsatellite markers from the entomopathogenic fungus Metarhizium anisopliae

Authors

  • JÜRG ENKERLI,

    Corresponding author
    1. Swiss Federal Research Station for Agroecology and Agriculture, Reckenholzstrasse 191, 8046 Zürich, Switzerland
      Jürg Enkerli, Fax: +41 (0)1 377 72 01; E-mail: juerg.enkerli@fal.admin.ch
    Search for more papers by this author
  • ROLAND KÖLLIKER,

    1. Swiss Federal Research Station for Agroecology and Agriculture, Reckenholzstrasse 191, 8046 Zürich, Switzerland
    Search for more papers by this author
  • SIEGFRIED KELLER,

    1. Swiss Federal Research Station for Agroecology and Agriculture, Reckenholzstrasse 191, 8046 Zürich, Switzerland
    Search for more papers by this author
  • FRANCO WIDMER

    1. Swiss Federal Research Station for Agroecology and Agriculture, Reckenholzstrasse 191, 8046 Zürich, Switzerland
    Search for more papers by this author

Jürg Enkerli, Fax: +41 (0)1 377 72 01; E-mail: juerg.enkerli@fal.admin.ch

Abstract

Fourteen polymorphic microsatellite markers were isolated from the entomopathogenic fungus, Metarhizium anisopliae, based on enriched genomic libraries. In order to assess allelic variability, the microsatellite loci were analysed in a collection of 34 isolates sampled from across Switzerland. The number of detected alleles in 14 loci ranged from two to eight and expected heterozygosity from 0.265 to 0.808. Because of the high expected heterozygosity, the 14 microsatellite loci are very useful for ecological studies and analysis of population diversity, and to identifying, monitoring, and tracking M. anisopliae strains applied as biological control agents.

Get access to the full text of this article

Ancillary