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Keywords:

  • bobcat;
  • dinucleotide repeats;
  • Lynx rufus;
  • microsatellites;
  • primers;
  • SSRs;
  • tetranucleotide repeats;
  • trinucleotide repeats

Abstract

We describe primers and polymerase chain reaction (PCR) conditions to amplify four dinucleotide, one trinucleotide, and three tetranucleotide microsatellite DNA loci from the bobcat (Lynx rufus). The primers were tested on 22 individuals collected from a population located within southwestern Georgia (USA). The primer pairs developed in this study yielded an average of 7.4 alleles per locus (range four to 10), an average observed heterozygosity of 0.60 (range 0.40 to 0.76), and an average polymorphic information content of 0.70 (range 0.51 to 0.78).