Isolation and characterization of microsatellite loci in the bee Ceratina flavipes

Authors


Noriko Azuma, Fax: +81-138-40-8864; E-mail: anoriko@fish.hokudai.ac.jp

Abstract

Polymorphic microsatellite markers can provide essential information on genetic analyses of sociality and mating behaviour of insects. We developed eight microsatellite loci in the small carpenter bee, Ceratina flavipes, using a magnetic bead hybridization enrichment protocol. These loci showed two to eight alleles with expected heterozygosity of 0.21–0.87, and also seemed useful to such congeneric species as Ceratina okinawana distributed in southern Japan. By using these loci, the sociality and mating systems in Ceratina species, that are still controversial, are possible to be analysed.

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