A set of chloroplast microsatellite primers for Eucalyptus (Myrtaceae)

  1. D. A. STEANE*,
  2. R. C. JONES,
  3. R. E. VAILLANCOURT

Article first published online: 9 MAY 2005

DOI: 10.1111/j.1471-8286.2005.00981.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 5, Issue 3, pages 538–541, September 2005

Additional Information

How to Cite

STEANE, D. A., JONES, R. C. and VAILLANCOURT, R. E. (2005), A set of chloroplast microsatellite primers for Eucalyptus (Myrtaceae). Molecular Ecology Notes, 5: 538–541. doi: 10.1111/j.1471-8286.2005.00981.x

Author Information

  1. Cooperative Research Centre for Sustainable Production Forestry, School of Plant Science, University of Tasmania, Private Bag 55, Hobart, Tasmania 7001, Australia

* D. A. Steane, Fax: +61 (0) 3-62262698; E-mail: Dorothy.Steane@utas.edu.au

Publication History

  1. Issue published online: 9 MAY 2005
  2. Article first published online: 9 MAY 2005
  3. Received 9 December 2004; revision accepted 25 February 2005

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Keywords:

  • Angophora;
  • Corymbia;
  • Melaleuca;
  • plastid;
  • Simple sequence repeat (SSR)

Abstract

We present a set of 35 chloroplast microsatellite primers for Eucalyptus. Ten of the microsatellites displayed intraspecific polymorphism, and identified nine haplotypes among 16 Eucalyptus globulus individuals. Primer conservation was high, with a polymerase chain reaction (PCR) success rate of 98% when tested on four other Eucalyptus species and seven additional myrtaceous genera. Chloroplast microsatellites have applications in phylogeographic studies, fingerprinting and progeny analysis.

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