Characterization and PCR multiplexing of polymorphic microsatellite loci for the locust Locusta migratoria

Authors

  • MARIE-PIERRE CHAPUIS,

    1. Centre de Biologie et de Gestion des Populations, INRA, Campus International de Baillarguet CS 30016, 34988 Montferrier/Lez, France,
    Search for more papers by this author
  • ANNE LOISEAU,

    1. Centre de Biologie et de Gestion des Populations, INRA, Campus International de Baillarguet CS 30016, 34988 Montferrier/Lez, France,
    Search for more papers by this author
  • YANNIS MICHALAKIS,

    1. Centre d’Etudes sur le Polymorphisme des Microorganismes, UMR CNRS-IRD 9926, Montpellier cedex 5, France,
    Search for more papers by this author
  • MICHEL LECOQ,

    1. Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Campus International de Baillarguet 34398 Montpellier cedex 5, France
    Search for more papers by this author
  • ARNAUD ESTOUP

    Corresponding author
    1. Centre de Biologie et de Gestion des Populations, INRA, Campus International de Baillarguet CS 30016, 34988 Montferrier/Lez, France,
    Search for more papers by this author

Errata

This article is corrected by:

  1. Errata: Corrigendum Volume 10, Issue 4, 755, Article first published online: 11 June 2010

Arnaud Estoup, Fax: +33 (0)499623345; E-mail: estoup@ensam.inra.fr

Abstract

Because of the scarcity of polymorphic genetic markers available in locust species, only a few population genetics studies have been carried out on this taxon. We isolated and characterized 11 polymorphic microsatellite loci in the pest locust Locusta migratoria capito, and described experimental conditions for polymerase chain reaction (PCR) multiplexing and simultaneously genotyping these loci. The number of alleles per locus ranged from six to 25, and the expected heterozygosity ranged from 0.431 to 0.957. Results of cross-taxon amplification tests are reported in six other Locusta migratoria subspecies, six species of the Oedipodinae subfamily and two other pest locust species.

Ancillary