Nematode-specific PCR primers for the 18S small subunit rRNA gene

Authors


Robin M. Floyd, Fax: (+44)1223 221259; E-mail: rmfl@bas.ac.uk

Abstract

A set of polymerase chain reaction primers were designed, which amplify a c. 1 kb fragment of the 18S ribosomal DNA gene, and are specific to the phylum Nematoda. These have proven useful in isolating nematode genes from samples mixed with other biological material, particularly with application to DNA barcoding. Optimal reaction conditions are described. These primers have successfully amplified the correct fragment from a wide phylogenetic range of nematodes, and have so far generated no sequences from any other organismal group.

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