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Isolation and characterization of microsatellite DNA markers in the Pacific abalone, Haliotis discus hannai

Authors

  • H. S. AN,

    1. Shellfish Genetics and Breeding Research Center, National Fisheries Research and Development Institute 421-2 Ongpo-ri, Hanlim-up, Bukjeju-gun, Jeju 695-835, Korea
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  • S. J. HAN

    1. Shellfish Genetics and Breeding Research Center, National Fisheries Research and Development Institute 421-2 Ongpo-ri, Hanlim-up, Bukjeju-gun, Jeju 695-835, Korea
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H. S. An, Fax: +82-64-796-1651; E-mail: hsan@nfrdi.re.kr

Abstract

We developed 17 new microsatellite markers in Haliotis discus hannai. All loci were found to be polymorphic with an average of 13.1 alleles per locus (range 3–28). The mean observed and expected heterozygosities were 0.77 (range 0.17–1.00) and 0.79 (range 0.42–0.96), respectively. Six loci deviated significantly from Hardy–Weinberg proportions, and thus should be used with caution. The high variabilities revealed in this study suggest that these microsatellite loci should provide useful markers for studies of trait mapping, kinship and population genetics.

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