Isolation and characterization of polymorphic microsatellite loci in the Hawaiian flycatcher, the elepaio (Chasiempis sandwichensis)

Authors

  • SARAH L. BURGESS,

    Corresponding author
    1. Evolution, Ecology, and Conservation Biology Program, Department of Cell and Molecular Biology, John A. Burns School of Medicine, 1960 East-West Road, University of Hawaii, Honolulu, HI 96822,
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  • ROBERT C. FLEISCHER

    1. Genetics Program, National Museum of Natural History, Smithsonian Institution, 3001 Connecticut Ave. NW, Washington, DC 20008, USA
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Sarah L. Burgess, slb@hawaii.edu

Abstract

Thirteen polymorphic microsatellite loci were isolated and characterized from two clades of an endemic Hawaiian flycatcher, the elepaio (Chasiempis sandwichensis). Seven dinucleotide repeats and one trinucleotide repeat were cloned from Kauai elepaio; five dinucleotide repeats were cloned from Oahu elepaio. Polymorphism was assessed in a sample of Oahu elepaio (n = 22) revealing two to 16 alleles per locus. Observed heterozygosity ranged from 0.14 to 0.91. However, linkage analysis exposed highly significant linkage disequilibrium between two of the most polymorphic loci. Twelve loci are therefore expected to be useful for investigations of population structure.

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