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Development and characterization of ten new microsatellite markers in a mangrove tree species Bruguiera gymnorrhiza (L.)

Authors

  • M. S. ISLAM,

    Corresponding author
    1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
    2. Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan,
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  • C. L. LIAN,

    1. Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan,
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  • N. KAMEYAMA,

    1. Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa, 903-0213, Japan
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  • B. WU,

    1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
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  • T. HOGETSU

    1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
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Md. Sajedul Islam, Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan Fax: +81-424-65-5601; E-mail: sajed@anesc.u-tokyo.ac.jp or sajed97@yahoo.com

Abstract

Bruguiera gymnorrhiza is an ecologically and somewhat economically important mangrove tree species. We isolated 10 polymorphic microsatellite loci from B. gymnorrhiza using a dual-suppression polymerase chain reaction (PCR) technique. These loci provided microsatellite markers with polymorphism of two to five alleles per locus within 216 individuals from nine natural populations of B. gymnorrhiza on Iriomote Island, the Sakishima Islands, Japan. The expected and observed heterozygosities ranged from 0.220 to 0.720 and from 0.104 to 0.447, respectively.

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