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Keywords:

  • Cahaba lily;
  • macrophyte;
  • Shoals spider lily;
  • SSR primers

Abstract

Microsatellite loci were isolated from the allotetraploid aquatic plant Hymenocallis coronaria. A repeat-enriched genomic library was constructed and primer pairs designed, resulting in six polymorphic loci. A total of 230 individuals were genotyped, and allelic richness per locus ranged from three to 11, while observed heterozygosity ranged from 0.017 to 0.570. Some amplified products were excised from agarose gel and sequenced to confirm primer specificity and mutation model. These are the first microsatellite markers developed for any member of this genus, and cross-amplification was successful with the only other member of the genus tested and with a member of the related genus Zephyranthes.