Development of microsatellite markers in Acer capillipes

Authors

  • H. TERUI,

    1. Laboratory of Forest Ecosystem Studies, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
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  • C. L. LIAN,

    1. Asian Natural Environmental Science Center, University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan
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  • Y. SAITO,

    Corresponding author
    1. Laboratory of Forest Ecosystem Studies, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
      Yoko Saito, Fax: +81-35841-8259; E-mail: yoko@es.a.u-tokyo.ac.jp
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  • Y. IDE

    1. Laboratory of Forest Ecosystem Studies, Graduate School of Agricultural and Life Sciences, University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
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Yoko Saito, Fax: +81-35841-8259; E-mail: yoko@es.a.u-tokyo.ac.jp

Abstract

Acer capillipes is an insect-pollinated tree species that grows in temperate regions of Japan. We isolated seven polymorphic microsatellite loci from this species using a dual-suppression polymerase chain reaction (PCR) technique. The number of alleles per locus ranged from two to 10, and the expected heterozygosity ranged from 0.042 to 0.828. Cross-species amplification from 14 other Acer species was successful for the majority of the isolated loci, suggesting that these loci may be useful for the characterization of other maple species.

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