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Development of microsatellite markers from an ectomycorrhizal fungus, Laccaria amethystina, by a dual-suppression-PCR technique

Authors

  • M. A. WADUD,

    Corresponding author
    1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
    2. Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan
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  • C. L. LIAN,

    1. Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan
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  • K. NARA,

    1. Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-shi, Tokyo 188-0002, Japan
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  • T. A. ISHIDA,

    1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
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  • T. HOGETSU

    1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo 113-8657, Japan,
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Md. Abdul Wadud, Fax: +81-424-65-5601; E-mail: wadud@anesc.u-tokyo.ac.jp, awadudaf@yahoo.com

Abstract

Laccaria amethystina is a major early successional ectomycorrhizal fungus. We isolated 10 polymorphic loci from L. amethystina using a dual-suppression-PCR (polymerase chain reaction) technique. These loci provided microsatellite markers with polymorphism of two to 10 alleles per locus. The observed and expected heterozygosities ranged from 0.136 to 0.545 and 0.206 to 0.877, respectively.

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