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Characterization of microsatellite markers in the endangered Sinojackia xylocarpa (Styracaceae) and cross-species amplification in closely related taxa

Authors


  • Ying Wang: equally contributed corresponding author

Hongwen Huang, Fax: 0086-27-87510251; E-mail: hongwen@wbgcas.cn

Abstract

Twenty-four polymorphic microsatellite loci were isolated and characterized from an AAG-enriched genomic library of Sinojackia xylocarpa. The average allele number of these microsatellites was 3.3 per locus, ranging from two to seven. The observed and expected heterozygosities at population level were 0.10–0.83 and 0.10–1.00, respectively. In addition, successful cross-species amplification of this set of microsatellites in three other species of Sinojackia and a closely related taxon, Changiostyrax dolichocarpa, suggested that this set of microsatellite markers should provide a useful tool for genetic and conservation studies of Sinojackia species and other closely related taxa in the Styracaceae.

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