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Keywords:

  • cross-species amplification;
  • cultivated yam;
  • Dioscorea trifida;
  • microsatellites

Abstract

We developed microsatellite markers in American yam (Dioscorea trifida). A microsatellite sequence-enriched genomic library was screened, and after sequencing, primers were designed for 20 microsatellites. Among these, eight primer pairs yielded amplification products that were both interpretable and polymorphic in 24 yam cultivars. The number of alleles per locus ranged from two to 13 and the overall expected heterozygosity was around 0.5. Six of the eight Dioscorea trifida microsatellite loci gave amplification products in other Dioscorea species.