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Isolation of polymorphic DNA microsatellites in the common sole Solea vulgaris

Authors

  • FLAVIO GAROIA,

    Corresponding author
    1. Molecular Genetics for Environmental and Fishery Resources Laboratory, Interdepartment Center of Research for Environmental Sciences, University of Bologna, Ravenna, Italy,
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  • SILLA MARZOLA,

    1. Molecular Genetics for Environmental and Fishery Resources Laboratory, Interdepartment Center of Research for Environmental Sciences, University of Bologna, Ravenna, Italy,
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  • ILARIA GUARNIERO,

    1. Aquaculture and Ichthyopathology Institute-Faculty of Veterinary Medicine, University of Bologna, Cesenatico (FC), Italy,
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  • MASSIMO TRENTINI,

    1. Aquaculture and Ichthyopathology Institute-Faculty of Veterinary Medicine, University of Bologna, Cesenatico (FC), Italy,
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  • FAUSTO TINTI

    1. Molecular Genetics for Environmental and Fishery Resources Laboratory, Interdepartment Center of Research for Environmental Sciences, University of Bologna, Ravenna, Italy,
    2. Marine Biology and Fisheries Laboratory, University of Bologna, Fano (PU), Italy
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Flavio Garoia, via S. Alberto 163, 48100 Ravenna, Italy. Fax: +39 544 937411; E-mail: garoia@cib.unibo.it

Abstract

A microsatellite-enriched genomic library was obtained from the common sole, Solea vulgaris, and seven polymorphic dinucleotide markers were successfully optimized. These markers showed levels of expected heterozygosity ranging from 0.55 to 0.83 and allele number ranging from eight to 12. Three of these markers were also found to successfully amplify in the closely related Solea senegalensis and can be employed to define population genetic structure of the two Solea species and for inferring stock origins.

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