Group-specific primers for amplifying DNA sequences that identify Amphipoda, Cephalopoda, Echinodermata, Gastropoda, Isopoda, Ostracoda and Thoracica

Authors

  • S. N. JARMAN,

    1. Department of the Environment and Heritage, Australian Antarctic Division, 203 Channel Highway, Kingston, TAS, 7050, Australia,
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  • K. S. REDD,

    1. Marine Research Laboratories, Tasmanian Aquaculture and Fisheries Institute, University of Tasmania, Private Bag 49, Hobart, Tasmania, 7000, Australia
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  • N. J. GALES

    1. Department of the Environment and Heritage, Australian Antarctic Division, 203 Channel Highway, Kingston, TAS, 7050, Australia,
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S. N. Jarman, Fax: (+61)362323449; E-mail: simon.jarman@aad.gov.au

Abstract

We describe seven group-specific primer pairs that amplify small sections of ribosomal RNA genes suitable for identification of animal groups of major importance as prey items in marine ecosystems. These primer sets allow the isolation of DNA from the target animal groups from mixed pools of DNA, where DNA-based identification using universal primers is unlikely to succeed. The primers are designed for identifying prey in animal diets, but could be used in any situation where these animal groups are to be identified by their DNA.

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