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Keywords:

  • Antarctic;
  • microsatellite;
  • Nototheniidae;
  • nuclear marker;
  • Trematomids

Abstract

Six polymorphic microsatellites were isolated from the dusky notothen, Trematomus newnesi (Boulenger 1902), using a microsatellite enrichment protocol and selective hybridization with di- tetra- and penta-repeat probes. The loci were screened in 48 individuals captured in the Southern Ocean (coastal zone of Terre-Adélie), revealing eight to 22 alleles per locus and an observed heterozygosity ranging from 0.70 to 0.92. These microsatellite markers provide a tool to study the relationship between the various morphs observed in this species and can be used for population genetic analysis and biodiversity studies.