Isolation and characterization of microsatellite loci from the ectomycorrhizal basidiomycete Suillus luteus

Authors

  • LUDO A. H. MULLER,

    1. Centre for Environmental Sciences, Environmental Biology Group, Universiteit Hasselt, Universitaire Campus, 3590 Diepenbeek, Belgium
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  • MARC LAMBAERTS,

    1. Centre for Environmental Sciences, Environmental Biology Group, Universiteit Hasselt, Universitaire Campus, 3590 Diepenbeek, Belgium
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  • JACO VANGRONSVELD,

    1. Centre for Environmental Sciences, Environmental Biology Group, Universiteit Hasselt, Universitaire Campus, 3590 Diepenbeek, Belgium
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  • JAN V. COLPAERT

    1. Centre for Environmental Sciences, Environmental Biology Group, Universiteit Hasselt, Universitaire Campus, 3590 Diepenbeek, Belgium
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Ludo A.H. Muller, Fax: +32 (0) 11 26 83 01; E-mail: ludo_muller@yahoo.com

Abstract

Eight microsatellite loci were isolated from the ectomycorrhizal basidiomycete Suillus luteus using a dual-suppression-polymerase chain reaction (PCR) method. In a test sample of 40 isolates, the total number of alleles per locus and the expected heterozygosities ranged from five to 16 and from 0.532 to 0.811, respectively. These highly polymorphic markers allow an accurate description of the genetic diversity and structure of S. luteus populations.

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