Isolation and characterization of microsatellite loci in the icefish Chionodraco rastrospinosus (Perciformes, Notothenioidea, Channichthyidae)

Authors

  • CHIARA PAPETTI,

    1. Department of Biology-University of Padova, via U. Bassi 58/B, I-35121 Padova, Italy,
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  • LORENZO ZANE,

    1. Department of Biology-University of Padova, via U. Bassi 58/B, I-35121 Padova, Italy,
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  • TOMASO PATARNELLO

    Corresponding author
    1. Department of Biology-University of Padova, via U. Bassi 58/B, I-35121 Padova, Italy,
    2. Department of Public Health, Comparative Pathology and Veterinary Hygene-University of Padova, Agripolis, via Romea 16, I-35020 Legnaro (Pd), Italy
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Tomaso Patarnello, Fax: +39 0498276209; E-mail: tomaso.patarnello@unipd.it

Abstract

We characterized eight polymorphic microsatellites in the icefish Chionodraco rastrospinosus (Perciformes, Notothenioidea, Channichthyidae). Microsatellites were isolated from a partial genomic library enriched for an AC motif. Chionodraco rastrospinosus is an endemic species inhabiting southern ocean waters surrounding the Antarctic Peninsula, the South Shetland Islands, and the South Orkney Islands. An excess of homozygotes was observed in seven out of the eight investigated loci; however, presence of null alleles was detected only for three of them suggesting that other factors may act in reducing heterozygosity. These molecular markers will be useful to investigate icefish genetic structure, possibly providing insights on its effective population size and demographic history.

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