Development and use of anchored-SSRs to study DNA polymorphism in bread wheat (Triticum aestivum L.)

Authors

  • RAVINDER SINGH,

    1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250 004 (U.P.), India
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  • NEERAJ KUMAR,

    1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250 004 (U.P.), India
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  • RAJIB BANDOPADHYAY,

    1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250 004 (U.P.), India
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  • SACHIN RUSTGI,

    1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250 004 (U.P.), India
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  • SHAILENDRA SHARMA,

    1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250 004 (U.P.), India
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  • HARINDRA SINGH BALYAN,

    1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250 004 (U.P.), India
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  • PUSHPENDRA KUMAR GUPTA

    1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut-250 004 (U.P.), India
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Pushpendra Kumar Gupta. Fax: +91-121-2768195; E-mail: pkgupta36@gmail.com

Abstract

In bread wheat, 21 anchored simple sequence repeat (SSR) primer pairs detecting SSR length polymorphism and 42 anchored SSR primers detecting microsatellite-anchored fragment length polymorphisms (MFLPs) are reported. Eight bread wheat genotypes were used for detecting polymorphism. The number of alleles in SSR analysis ranged from two to six, with a mean of 2.9 alleles per SSR. The number of polymorphic bands in MFLP ranged from two to 40, with a mean of 12.74 polymorphic bands/primer combination, the SSRs with CT/GA motifs giving the highest level of polymorphism (a mean of 18.37 bands). The average value of polymorphic information content (PIC) was 0.473 for SSRs and 0.061 for MFLP.

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