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Identification and characterization of simple sequence repeat (SSR) markers from Fragaria×ananassa expressed sequences

Authors

  • ANDREW KENIRY,

    1. Primary Industries Research Victoria – Bundoora Centre, Victorian AgriBiosciences Centre, 1 Park Drive, Bundoora, Victoria 3083, Australia,
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  • CLARE J. HOPKINS,

    1. Primary Industries Research Victoria – Bundoora Centre, Victorian AgriBiosciences Centre, 1 Park Drive, Bundoora, Victoria 3083, Australia,
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  • ERICA JEWELL,

    1. Primary Industries Research Victoria – Bundoora Centre, Victorian AgriBiosciences Centre, 1 Park Drive, Bundoora, Victoria 3083, Australia and Victorian Bioinformatics Consortium,
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  • BRUCE MORRISON,

    1. Department of Primary Industries — Knoxfield, Private Mail Bag 15, Ferntree Gully Delivery Centre, Knoxfield, Victoria 3156, Australia
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  • GERMAN C. SPANGENBERG,

    1. Primary Industries Research Victoria – Bundoora Centre, Victorian AgriBiosciences Centre, 1 Park Drive, Bundoora, Victoria 3083, Australia and Victorian Bioinformatics Consortium,
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  • DAVID EDWARDS,

    1. Primary Industries Research Victoria – Bundoora Centre, Victorian AgriBiosciences Centre, 1 Park Drive, Bundoora, Victoria 3083, Australia and Victorian Bioinformatics Consortium,
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  • JACQUELINE BATLEY

    1. Primary Industries Research Victoria – Bundoora Centre, Victorian AgriBiosciences Centre, 1 Park Drive, Bundoora, Victoria 3083, Australia,
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Jacqueline Batley, Fax: +61 (0)3 9479 3168; E-mail: jacqueline.batley@dpi.vic.gov.au

Abstract

The availability of expressed sequence data derived from gene discovery programmes enables mining for simple sequence repeats (SSRs), providing useful genetic markers for crop improvement. These markers are inexpensive, require minimal labour to produce and can frequently be associated with functionally annotated genes. This study reports on the development and characterization of expressed sequence tag (EST)–SSR markers in the cultivated strawberry, Fragaria×ananassa. Fourteen primer pairs were assessed for polymorphism in 13 F.×ananassa genotypes. The markers show reliable amplification and considerable polymorphism, demonstrating the utility of EST–SSRs for genetic analysis of commercial strawberry germplasm.

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