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Isolation and characterization of microsatellite loci in Cylindrocladium parasiticum

Authors


Louwrance P. Wright, Fax: +27124203960; E-mail: lowrie.wright@fabi.up.ac.za

Abstract

Twelve polymorphic microsatellite markers were developed for Cylindrocladium parasiticum, a plant pathogen with a wide host range and the causal agent of the serious disease of peanuts (Arachis hypogaea) known as cylindrocladium black rot (CBR). Polymorphism was evaluated on 17 isolates from different hosts and regions. Each locus had between two and six alleles. Cross-species transferability tested for 20 other Cylindrocladium species found amplification only in Cylindrocladium pacificum, which is phylogenetically closely related to C. parasiticum.

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