Factors affecting success of PCR amplification of microsatellite loci from otter faeces

  1. P. HÁJKOVÁ1,2,
  2. B. ZEMANOVÁ1,2,
  3. J. BRYJA1,2,
  4. B. HÁJEK3,
  5. K. ROCHE4,5,
  6. E. TKADLEC1,6,
  7. J. ZIMA1

Article first published online: 28 APR 2006

DOI: 10.1111/j.1471-8286.2006.01269.x

Issue

Molecular Ecology Notes

Molecular Ecology Notes

Volume 6, Issue 2, pages 559–562, June 2006

Additional Information

How to Cite

HÁJKOVÁ, P., ZEMANOVÁ, B., BRYJA, J., HÁJEK, B., ROCHE, K., TKADLEC, E. and ZIMA, J. (2006), Factors affecting success of PCR amplification of microsatellite loci from otter faeces. Molecular Ecology Notes, 6: 559–562. doi: 10.1111/j.1471-8286.2006.01269.x

Author Information

  1. 1

    Institute of Vertebrate Biology, Academy of Sciences of the Czech Republic, Květná 8, 603 65 Brno, Czech Republic,

  2. 2

    Department of Zoology and Ecology, Faculty of Science, Masaryk University, Kotlářská 2, 611 37 Brno, Czech Republic,

  3. 3

    Administration of the Slovenský Raj National Park, State Nature Conservancy of the Slovak Republic, Letecká 3, 052 01 Spišská Nová Ves, Slovak Republic,

  4. 4

    Czech Otter Foundation Fund, P.O. Box 53, 379 01 Třeboň, Czech Republic,

  5. 5

    Environment Agency, Bromholme Lane, Brampton, Huntingdon, Cambridgeshire, PE28 4NE, UK,

  6. 6

    Department of Ecology and Environmental Sciences, Faculty of Science, Palacky University, tř. Svobody 26, 771 46 Olomouc, Czech Republic

* P. Hájková, Fax: +420-543211 346; E-mail: hajkova@ivb.cz

Publication History

  1. Issue published online: 28 APR 2006
  2. Article first published online: 28 APR 2006
  3. Received 2 November 2005; revision accepted 1 December 2005

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Keywords:

  • amplification success;
  • faecal DNA;
  • Lutra;
  • noninvasive sampling

Abstract

We investigated the effect of multiple variables on the amplification success rate of microsatellite DNA extracted from faeces of wild Eurasian otters. The success rate was affected by (i) type of sample, with higher success rates in anal jelly samples than faeces, and (ii) temperature, with a negative effect of increased temperature at time of collection. To increase the effectiveness of microsatellite genotyping of otter faeces, we recommend collecting samples in cold months and early in the morning, preferably in a frozen state, and the collection of anal jelly samples, or the jelly part from faeces, whenever possible.

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