Get access

Polymorphic microsatellite loci from the West Nile virus vector Culex tarsalis

Authors

  • JASON L. RASGON,

    1. The W. Harry Feinstone Department of Molecular Microbiology and Immunology, and
    2. The Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Suite E5132 N. Wolfe Street, Baltimore, Maryland 21205, USA
    Search for more papers by this author
  • MEERA VENKATESAN,

    1. The W. Harry Feinstone Department of Molecular Microbiology and Immunology, and
    2. The Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Suite E5132 N. Wolfe Street, Baltimore, Maryland 21205, USA
    Search for more papers by this author
  • CATHERINE J. WESTBROOK,

    1. The W. Harry Feinstone Department of Molecular Microbiology and Immunology, and
    2. The Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Suite E5132 N. Wolfe Street, Baltimore, Maryland 21205, USA
    Search for more papers by this author
  • MARY CLAIRE HAUER

    1. The W. Harry Feinstone Department of Molecular Microbiology and Immunology, and
    2. The Johns Hopkins Malaria Research Institute, Bloomberg School of Public Health, Johns Hopkins University, Suite E5132 N. Wolfe Street, Baltimore, Maryland 21205, USA
    Search for more papers by this author

Jason L. Rasgon, Fax: 1-410-955-0105; E-mail: jrasgon@jhsph.edu

Abstract

Since its introduction in 1999, West Nile virus (WNV) has spread across North America. Culex tarsalis is a highly efficient WNV vector species. Many traits such as virus susceptibility, autogeny and host preference vary geographically and temporally in C. tarsalis. Culex tarsalis genomic libraries were developed and were highly enriched for microsatellite inserts (42–96%). We identified 12 loci that were polymorphic in wild C. tarsalis populations. These microsatellites are the first DNA-based genetic markers developed for C. tarsalis and will be useful for investigating population structure and constructing genetic maps in this mosquito.

Ancillary