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Variable nuclear markers for Melanoplus oregonensis identified from the screening of a genomic library

Authors

  • B. C. CARSTENS,

    1. Department of Ecology and Evolutionary Biology, 1109 Geddes Rd., Museum of Zoology, Rm. 1089, University of Michigan, Ann Arbor, Michigan 48109-1079, USA
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  • L. L. KNOWLES

    1. Department of Ecology and Evolutionary Biology, 1109 Geddes Rd., Museum of Zoology, Rm. 1089, University of Michigan, Ann Arbor, Michigan 48109-1079, USA
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Bryan C. Carstens, Fax: (734) 763-4080; E-mail: bcarsten@umich.edu

Abstract

We report the isolation of seven single-copy nuclear loci from the montane grasshopper Melanoplus oregonensis. With an average length of approximately 930 bp and between five and 67 variable sites, these loci are useful for population genetic analyses within M. oregonensis. Amplification of nuclear loci in additional species of western Melanoplus grasshoppers suggests that they will also be useful for a variety of population genetic, phylogeographic and phylogenetic studies.

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