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Keywords:

  • 5′-nuclease reaction;
  • coho salmon;
  • Oncorhynchus kisutch;
  • SNP;
  • TaqMan

Abstract

We report 39 single nucleotide polymorphisms (SNPs) observed in 23 nuclear DNA sequences in coho salmon Oncorhynchus kisutch. High-throughput genotyping assays based on the 5′-nuclease reaction were developed for 17 of these nuclear SNPs and for two previously published mitochondrial DNA SNPs. Minor allele frequency differences (Δq) among collections were between 5.2% and 51.2%, resulting in per locus FST estimates of 0.00–0.24 with an average of 0.09.