Development of microsatellite markers in the toxic dinoflagellate Alexandrium minutum (Dinophyceae)

Authors

  • S. NAGAI,

    1. National Research Institute of Fisheries and Environment of Inland Sea, Harmful Algal Bloom Division, Toxic Phytoplankton Section, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan,
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  • L. MCCAULEY,

    1. Woods Hole Oceanographic Institution, MS 32, Redfield 332, Woods Hole, Massachusetts 02543-1049, USA,
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  • N. YASUDA,

    1. Graduate School of Information Science and Engineering, Tokyo Institute of Technology, 2-12-1 O-okayama, Meguro-ku, Tokyo 152-8552, Japan
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  • D. L. ERDNER,

    1. Woods Hole Oceanographic Institution, MS 32, Redfield 332, Woods Hole, Massachusetts 02543-1049, USA,
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  • D. M. KULIS,

    1. Woods Hole Oceanographic Institution, MS 32, Redfield 332, Woods Hole, Massachusetts 02543-1049, USA,
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  • Y. MATSUYAMA,

    1. National Research Institute of Fisheries and Environment of Inland Sea, Harmful Algal Bloom Division, Toxic Phytoplankton Section, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan,
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  • S. ITAKURA,

    1. National Research Institute of Fisheries and Environment of Inland Sea, Harmful Algal Bloom Division, Toxic Phytoplankton Section, 2-17-5 Maruishi, Hatsukaichi, Hiroshima 739-0452, Japan,
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  • D. M. ANDERSON

    1. Woods Hole Oceanographic Institution, MS 32, Redfield 332, Woods Hole, Massachusetts 02543-1049, USA,
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S. Nagai, Fax: +81-829-54-1216; E-mail: snagai@affrc.go.jp

Abstract

Outbreaks of paralytic shellfish poisoning caused by the toxic dinoflagellate Alexandrium minutum (Dinophyceae) are a worldwide concern from both the economic and human health points of view. For population genetic studies of A. minutum distribution and dispersal, highly polymorphic genetic markers are of great value. We isolated 12 polymorphic microsatellites from this cosmopolitan, toxic dinoflagellate species. These loci provide one class of highly variable genetic markers, as the number of alleles ranged from four to 12, and the estimate of gene diversity was from 0.560 to 0.862 across the 12 microsatellites; these loci have the potential to reveal genetic structure and gene flow among A. minutum populations.

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