Ten microsatellite primer pairs, developed from Caulanthus amplexicaulis var. barbarae (J. Howell) Munz, specifically amplified target loci across a diverse range of taxa from the Streptanthoid Complex, Brassicaceae. All primer pairs produced amplification products from at least 22 of the 23 accessions tested. Product size variation and observable heterozygosity were consistent with what would be expected from the amplification of polymorphic microsatellites across the complex. Our findings demonstrate that microsatellites developed for C. amplexicaulis var. barbarae will be useful for population-genetic studies in taxa throughout the Streptanthoid Complex.