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Novel primers for detection and quantification of Myxococcus species in situ
Article first published online: 20 JUL 2006
DOI: 10.1111/j.1471-8286.2006.01339.x
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How to Cite
MARTIN, K. J. and BULL, C. T. (2006), Novel primers for detection and quantification of Myxococcus species in situ. Molecular Ecology Notes, 6: 773–775. doi: 10.1111/j.1471-8286.2006.01339.x
Publication History
- Issue published online: 14 AUG 2006
- Article first published online: 20 JUL 2006
- Received 5 January 2006; revision accepted 22 January 2006
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Keywords:
- myxobacteria;
- quantitative PCR
Abstract
A nested polymerase chain reaction (PCR) protocol using unique primers was developed to detect and quantify Myxococcus species from environmental samples. The protocol amplified most species of Myxococcus when 10 pg of DNA representing 1000 cells was present, although over half were amplified with as little as 0.1 pg (10 cells). The protocol did not amplify other myxobacterial species, members of the δ-proteobacteria or other unrelated organisms tested at significantly higher concentrations of DNA. The primers were also used in quantitative PCRs, which accurately estimated the population levels in soil.