Present address: Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA, and CIBIO, Centro de Investigação em Biodiversidade e Recursos, Genéticos Campus Agrário de Vairão Universidade do Porto, 4485-601 Vairão, Portugal.
Sequencing primers and SNPs for rapidly evolving reproductive loci in endangered ibex and their kin (Bovidae, Capra spp.)
Article first published online: 23 MAY 2006
Molecular Ecology Notes
Volume 6, Issue 3, pages 776–779, September 2006
How to Cite
JORDAN, S., MIQUEL, C., TABERLET, P. and LUIKART, G. (2006), Sequencing primers and SNPs for rapidly evolving reproductive loci in endangered ibex and their kin (Bovidae, Capra spp.). Molecular Ecology Notes, 6: 776–779. doi: 10.1111/j.1471-8286.2006.01340.x
- Issue published online: 14 AUG 2006
- Article first published online: 23 MAY 2006
- Received 2 September 2005; revision received 30 January 2006; accepted 7 February 2006
- candidate genes;
- molecular adaptation;
Rapidly evolving genes (e.g. candidate selected loci) are of increasing interest to molecular ecologists and conservation geneticists. Here, we report primers for five regions from three independent nuclear reproductive genes that reliably generate polymorphic sequences across the widespread wild goats of the Capra ibex species group and likely many other species of bovids. From three to nine single-nucleotide polymorphisms (SNPs) were identified in each gene region among C. ibex subspecies. Average numbers of SNPs per 1000 bp across all five gene regions was 15.0, with a high of 25.3 in the ZP3 exons 3 and 4 sequence and a low of 6.1 in the TNP1 sequence.