Sequencing primers and SNPs for rapidly evolving reproductive loci in endangered ibex and their kin (Bovidae, Capra spp.)

Authors

  • STEVE JORDAN,

    1. Department of Biology, Bucknell University, Lewisburg, PA 17837, USA,
    2. Laboratoire d’Ecologie Alpine, Génomique des Populations et Biodiversité, CNRS UMR 5553, Université Joseph Fourier, BP 53, F-38041 Grenoble, Cedex 9, France
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  • CHRISTIAN MIQUEL,

    1. Laboratoire d’Ecologie Alpine, Génomique des Populations et Biodiversité, CNRS UMR 5553, Université Joseph Fourier, BP 53, F-38041 Grenoble, Cedex 9, France
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  • PIERRE TABERLET,

    1. Laboratoire d’Ecologie Alpine, Génomique des Populations et Biodiversité, CNRS UMR 5553, Université Joseph Fourier, BP 53, F-38041 Grenoble, Cedex 9, France
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  • GORDON LUIKART

    1. Laboratoire d’Ecologie Alpine, Génomique des Populations et Biodiversité, CNRS UMR 5553, Université Joseph Fourier, BP 53, F-38041 Grenoble, Cedex 9, France
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    • Present address: Division of Biological Sciences, University of Montana, Missoula, MT 59812, USA, and CIBIO, Centro de Investigação em Biodiversidade e Recursos, Genéticos Campus Agrário de Vairão Universidade do Porto, 4485-601 Vairão, Portugal.


Steve Jordan, Fax: 570 577-3537; E-mail: sdjordan@bucknell.edu

Abstract

Rapidly evolving genes (e.g. candidate selected loci) are of increasing interest to molecular ecologists and conservation geneticists. Here, we report primers for five regions from three independent nuclear reproductive genes that reliably generate polymorphic sequences across the widespread wild goats of the Capra ibex species group and likely many other species of bovids. From three to nine single-nucleotide polymorphisms (SNPs) were identified in each gene region among C. ibex subspecies. Average numbers of SNPs per 1000 bp across all five gene regions was 15.0, with a high of 25.3 in the ZP3 exons 3 and 4 sequence and a low of 6.1 in the TNP1 sequence.

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